Plasmid Isolation Lab Write Up

This is a lab write-up.  Please go based on the rubric I will have copied here.  I will attach the rubric and everything related to this lab write-up that can be used for this assignment.   I have the limit set for 3 pages.  Please let me know if requires fewer pages or maybe 4.  I have it as 3.  I will also attach the Gel-Electrophoresis picture that I took when I did the lab.  This will be also included in the write-up.  The lab write-up is due on Sunday the 31st of October.  I have the deadline set for the 29th so I can submit this as a rough draft and get a response back from the professor so we can fix and turn it finally on the deadline.  I want to get 100% on this.  The professor will not accept a rough draft beyond the 29th.  Please beware that this will be submitted via TurnItIn for any type of plagiarism.  Please let me know if you have any questions.

Write-up #3 (30 points) Date: _____________ Summary for Name of Lab/ExperimentSection I. Introduction (4 paragraphs) 13 pts:Paragraph 1: General paragraph about what this experiment is about1. Start with a general statement about the experiment 2. What specific methods are used here? Paragraph 2: Articulate the problemwhy is miniprep a better technique than others to isolate plasmid DNA?1. Why is this method, the miniprep, used to isolate plasmid DNA? 2. How does this method compare to other methods to isolate DNA?(Include at least one citation to support your statement).Paragraph 3: ImportanceWhy are these techniques (miniprep and RE) important?1. Describe plasmids and what they are used for in research? 2. Describe restriction enzymes and what they are used for in research?(Include at least one citation to support your statements).Paragraph 4: Gist of what we are doingWhat specifically are we doing/investigating here?Include the following points (very briefly, 1-2 sentences per point) to describe what is going to be done; this is NOT a Material and Methods, rather a brief description of the approach for the experiment.1. specifics about which plasmid is being extracted from which bacterium2. which instrument is being used for quantifying plasmid DNA 3. Which enzyme is being used to cut plasmid DNA4. What method will be used to visualize plasmid DNA? Section II. Results (2 sentences) 2 pts:1. What are the key findings of your experiments? (you may have more than 1 key result from your weeks experiments) Figure, as per guidelines provided 4 ptsSection III. Discussion (2 sentences) 2 pts:1. What are the conclusions of the weeks study/experiment? 2. Clearly state the implications of the results that you got and frame it in the context of why the experiment is important.

Section IV. Work Cited 4 pts:1. At least two citations. Formatted as in the journal Cell. Fonts, Grammatical, style 5 pts:1. Write concisely (do not include unnecessary details)2. Write in past tense (except the implications and trends in the discussion section)3. 1-inch margins on left and right side.4. Use Times New Roman (size between 10 to 12 points) for the text and Arial for the figures and figure legend (font size =8)5. Make your figure on a separate page. Must include figure number, title, and legend as per instructions for figures and tables in the writing guidelines.

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