Assignment: Case Study DNA
Tube Sample Lane
1 1 kb DNA ladder 1
2 Mother’s DNA 2
3 Child’s DNA 3
4 A.F. #1 DNA 4
5 A.F. #2 DNA 5
Record the distance each ladder band moved from the well in mm along with the size of the DNA fragments in that band in bp units, based on the bp given in step 19 , in your Lab Notes.
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Lane 1:
1,000 bp 34 mm
900 bp 36 mm
800 bp 38 mm
700 bp 40 mm
600 bp 43 mm
500 bp 47 mm
400 bp 51 mm
300 bp 56 mm
250 bp 60 mm
200 bp 64 mm
150 bp 69 mm
100 bp 77 mm
50 bp 90 mm
Lane 2:
37 mm 850bp
59 mm 265bp
Lane 3:
37 mm 850bp
44 mm 575bp
Lane 4:
41 mm 670bp
43 mm 600bp
Lane 5:
44 mm 575bp
55 mm 320bp
| DNA Ladder | |
|---|---|
| Band | Distance (mm) |
| 50 bp | 90 mm |
| 100 bp | 77 mm |
| 150 bp | 69 mm |
| 200 bp | 64 mm |
| 250 bp | 60 mm |
| 300 bp | 56 mm |
| 400 bp | 51 mm |
| 500 bp | 47 mm |
| 600 bp | 43 mm |
| 700 bp | 40 mm |
| 800 bp | 38 mm |
| 900 bp | 36 mm |
| 1,000 bp | 34 mm |
| A.F. #2 |
| 41 mm 670bp
43 mm 600bp |
| 37 mm 850bp |
| A.F. #2 |
|
DNA has genetic information that controls our cells. So, DNA is like a blueprint that shows how to construct components of cells like proteins and ribonucleic acid (RNA). This information is carried down to newer generations through inheritance. |
| In order to make DNA visible. The Gel has to be soaked in a dye (ethidium bromide) to bind with the DNA and rinsed off after. Ethidium bromide helps to make DNA visible by glowing brightly in UV rays. |
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