Grossmont College Biuret Assay for Protein Case Study

Question Description

Activity 1: Biuret Assay for Protein

Table 1: Biuret Assay for Protein

Your Prediction

Actual Assay Results Description

Protein Present? (Yes/No)

Nutrition Label Information

PositiveControl (BSA)

Purple

Negative Control (Water)

Light blue

Milk

purple

Apple Juice

Light blue

Potato flakes

Light blue

Gelatin

purple

Egg White

purple

Notes:

Activity 2:Lugol’s Iodine Test for Starch

Table 2: Lugol’s Test for Starch

Prediction

Assay Results Description

Starch Present? (Yes/No)

Nutrition Label Information

Positive Control (1% corn starch)

Dark Blue/black

Negative Control (Water)

golden

Milk

golden

Apple Juice

golden

Potato flakes

Dark blue/black

Gelatin

golden

Egg White

golden

Notes:

Activity 3: Benedict’s Test for Reducing Sugars

Table 3: Benedicts Test for Reducing Sugars

Prediction

Assay Results Description

Sugar Present? (Yes/No)

Nutrition Label Information

Positive Control (10% Glucose)

Brick red color

Negative Control (Water)

Light blue

Milk

Orange-red color

Apple Juice

Orange-red color

Potato flakes

Yellow-green

Gelatin

Light blue

Egg White

Light blue

Notes:

Activity 4: Paper Test for Lipids

Table 4: Paper Test for Lipids

Prediction

Assay Results Description

Lipids Present? (Yes/No)

Nutrition Label Information

Positive Control (Corn Oil)

Greasy spot

Negative Control (Water)

No spot

Milk

Slightly greasy spot

Apple Juice

No spot

Potato flakes

No spot

Gelatin

No spot

Egg White

No spot

Notes:

Activity 5: Summary of Results

Table 5: Summary of Biological Molecules in Foods

Biuret test for Protein

Lugol’s test for starch

Benedicts test for reducing sugars

Paper test for lipids

Milk

Apple Juice

Potato flakes

Gelatin

Egg White

Activity 6: Communicating Your Results

In the space below, summarize the results of your tests. Did your results match your predictions? Were there any cases where you were surprised at the results you obtained? How did your results compare to the information from the nutritional data sheets?

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