Experiment (dry lab)
ocular lenshead
objective lensstage
condenser lens with filterlight
revolving nosepiecebase
coarse adjustment knobrheostat
fine adjustment knobarm
iris diaphragm
|
A |
Eyepiece |
|
|
B |
Supports the ocular lenses |
|
|
C |
Holds objectives that can be rotated |
|
|
D |
Connects the base and barrel |
|
|
E |
Lens that magnifies the specimen |
|
|
F |
Supports the slide |
|
|
G |
Focuses the light onto the specimen |
|
|
H |
Raises and lowers the stage for focusing |
|
|
I |
Slightly moves the stage to sharpen the image |
|
|
J |
Illuminator |
|
|
K |
Light control |
|
|
L |
Supports the microscope |
|
|
M |
Varies the intensity of the light |
Experiment (dry lab)
Field of view
|
Lens |
Lens Power |
Ocular lens |
Total Magnification |
Diameter in mm (3 significant figures) |
Diameter in mm (3 sig figs) |
Area in mm2 A = pr2 (p = 3.14) (3 sig figs) |
|
Scan Power |
4X |
10X |
40X |
4.200 |
4200 |
13.85 |
|
Low Power |
10X |
|||||
|
HighPower |
40X |
|||||
|
Oil Immersion |
100X |
Note:
What happens to the field of view as magnification increases?
(It increase, decrease or stay the same.)
–Depth of focus
What happens to the depth of focus as magnification increases?
(It increase, decrease or stay the same.).
–Image orientation
Select the letter e as you would see through the ocular if it were mounted right side up on the slide.(A, B, C or D.)
II. Cytology
Experiment (dry lab)
A.Wet Mounts
B.Eukaryotes
|
A |
|
|
B |
|
|
C |
|
|
D |
|
A |
|
|
B |
|
|
C |
|
|
D |
|
|
E |
|
A |
|
|
B |
|
|
C |
C.Prokaryotes
Identify these bacterial cells as coccus, bacillus or spirillum
|
A |
|
|
B |
|
|
C |
D. Cell Models: Label the animal and plant cells.
Animal cell model:
Label – centrioles (pair), cytoplasm, Golgi apparatus, mitochondria, nucleus and plasma membrane.
|
Animal cell model #1 |
||
|
A |
Boundary between cytosol and extracellular fluid |
|
|
B |
Flattened stacked discs |
|
|
C |
Synthesize ATP |
|
|
D |
Cytosol + organelles |
|
|
E |
Found in pairs, made of microtubules |
|
|
F |
Holds the DNA |
|
Nucleus:
Label -DNA (chromatin), nuclear membrane, nuclear pore and nucleolus.
|
Animal cell/Plant cell Model #2 |
||
|
A |
Double membrane |
|
|
B |
Assemble ribosomal subunits |
|
|
C |
Genetic material |
|
|
D |
Perforation on the nuclear membrane |
|
Plant cell:
Label cell wall, central vacuole, chloroplasts, cytoplasm, Golgi apparatus, plasma membrane, mitochondria and nucleus.
|
Plant cell Model #3 |
||
|
A |
Boundary between cytosol and cell wall |
|
|
B |
Synthesis of ATP |
|
|
C |
Flattened stacked discs |
|
|
D |
Supports the cell |
|
|
E |
Holds the DNA |
|
|
F |
Cytosol + organelles |
|
|
G |
Membranous sac stores water + other substances |
|
|
H |
Site for photosynthesis |
|
End of Lab J
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